Evaluation of inhibitory effect of trichoderma strain on strawberry colletotrichum gloeosporioides

Fermentation broth from richoderma viride strain HZ0501 was prepared by biological fermentation.Antifungal activity of fermentation broth evaluation was carried out by the target bacteria against Colletotrichum Gloeosporioides.The results showed that fermentation broth had significant activity against Colletotrichum Gloeosporioides.After being treated by ultrasonic wave for 12 minutes,the fermentation broth from original broth showed significantly higher antifungal activity,suggesting that intra-and exta-cellular of Trichoderma koningii strain had antifungal substances.Antifungal substances were stable at high temperature and different pH value.The fermentation broth that treated by at 60 ℃ and acid pH 5 significantly increased activity against Colletotrichum Gloeosporioides.Compared with undiluted control,fermentation broth that was diluted 100 fold with water significantly improved activity against Colletotrichum Gloeosporioides.In addition,fermentation broth had higher activity of cellulose and β-glucanase.