Epidemiological Investigation of the Prevalence of High Risk Human Papillomavirus and Its Correlation with Cervical Intraepithelial Neoplasia in 4874 Women in Guangxi Province

Objective:To investigate the prevalence of high risk human papillomavirus (HPV) infection and its correlation with Cervical Intraepithelial Neoplasia (CIN) in Guangxi province. Methods:HPV in 4874 women was detected by hybrid capture Ⅱ (HC2) or HPV genotyping assay. High-risk HPV samples were detected by cervical biopsy.Results:①Detection by two methods: HPV positive rate was 27.00%(1316/4874). Positive rate of high-risk HPV infection was 23.78% (1159/4874), low-risk HPV was 3.22%(157/4874). In high risk HPV infection, 57 was CIN, account for 4.92% (57/1159), in which CINⅡ～Ⅲ were 29 cases,account for 2.50%(29/1159)②Testing results by HC2: Among 2260 patients, the positive incidence of high-risk HPV infection was 24.96% (564/2260), in which 29 patients were detected as CIN, account for 5.14%(29/564), and 15 cases were detected as CINⅡ～Ⅲ, account for 2.66% (15/564). There was statistical difference about viral loading dose between CINⅠ～Ⅲ with less than 100 ng/L and more than 100 ng/L(χ2=21.098,P=0.000). ③ Results of HPV genotyping assay: Among 2614 patients, HPV positive rate was 28.77%(752/2614), while the positive rate was 22.76%(595/2614)in high-risk HPV infection and 6.01%(157/2614)in low-risk HPV infection. 28 patients were CIN in high risk HPV infection by genotype, account for 4.71%(28/595). 14 cases were detected as CINⅡ～Ⅲ, with a rate of 2.35%(14/595). 3 cases were detected as CIN in low-risk HPV infection, account for 1.91%(3/157). There was no high-grade lesion. The positive rate of HPV detection in genotyping assay group was 27.56% in type 16, 25.21% in type 52, and 21.85% in type 58, respectively. Conclusions:The positive rate of cervical high-risk HPV infection and detection rate of cervical high-grade lesion in positive HPV infection are higher in Guangxi province. HC2 test indicates that the higher the HPV viral loading dose, the higher the detection rate of high-grade lesion. HPV genotypes 16, 52 and 58 are the commonest high-risk genotypes in this area.