Effect of dihydroarteannuin on metastatic tumor growth in Lewis lung cancer bearing C57BL/6J mice

OBJECTIVE:To explore the anti-tumor effect and the mechanism of dihydroarteannuin(DHA)in lung cancer bearing C57BL/6J mice.METHODS:Fifty C57BL/6J mice subcutaneous planted with 3LL lung cancer cells(2×106)were randomly divided into 5 groups:a blank control group with same volume of normal saline;positive control DDP group;low,middle and high dose groups.The body weight changes and inhibitory rate of the mice in each group were observed.In addition,the cell cycle and apoptosis index were analysed by flow cytometry.RESULTS:The body weights were decreased in the middle and high dose groups vs the NS group.Compare with the NS group,the inhibitory rate of the low dose group was 7.01%,there was non-significantly difference(t=0.872,P=0.395),and at the same time,in the middle and high dose groups the inhibitory rates were 53.50% and 59.24% respectively which were lower than that of DDP group(92.3%;t=-0.871,P=0.000;t=-9.667,P=0.000),and there were significant differences(t=8.541,P=0.000;t=13.566,P=0.000).The FCM assay indicated that DHA induced the apoptosis of lung cancer cells.At the same time,the number of G0/G1 and G2/M phase cells were significantly decreased,most of the cells were significantly arrested in S phase compared with the NS group,P=0.000.CONCLUSION:DHA is provided with the anti-tumor effect to lung cancer bearing C57BL/6J mice at certain range of dosage.Its mechanism might relate to inducing cancer cell apoptosis.