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2007•Journal of Southwest UniversityRequires access

Separation and Identification of a New Taxus chinensis var. mairei Endophytic Fungus (Bionectria sp.) and the Activity of Its Metabolites

Changhua Hu

Open publisher page 5 citations

Abstract

Thirty endophytic fungi have been isolated from the bark of Taxus chinensis var.mairei.Morphologic and ITS-5.8S rDNA sequence identification methods used in combination identified one of them(XH004)to be Bionectria sp.,which is a newly-recorded species in Taxaceae endophytic fungi.Bacteriostatic test,cytotoxic test,thin layer chromatography(TLC)and high performance liquid chromatography(HPLC)confirmed that the metabolites of XH004 had obvious bacteriostatic and cytocidal activities and suggested that they probably contain certain taxol or taxol analog.

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What this paper is about

Thirty endophytic fungi have been isolated from the bark of Taxus chinensis var.mairei.Morphologic and ITS-5.8S rDNA sequence identification methods used in combination identified one of them(XH004)to be Bionectria sp.,which is a newly-recorded species in Taxaceae endophytic fungi.Bacteriostatic test,cytotoxic test,thin layer chromatography(TLC)and high performance liquid chromatography(HPLC)confirmed that the metabolites of XH004 had obvious bacteriostatic and cytocidal activities and suggested that they probably contain certain taxol or taxol analog.

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Available abstract

Thirty endophytic fungi have been isolated from the bark of Taxus chinensis var.mairei.Morphologic and ITS-5.8S rDNA sequence identification methods used in combination identified one of them(XH004)to be Bionectria sp.,which is a newly-recorded species in Taxaceae endophytic fungi.Bacteriostatic test,cytotoxic test,thin layer chromatography(TLC)and high performance liquid chromatography(HPLC)confirmed that the metabolites of XH004 had obvious bacteriostatic and cytocidal activities and suggested that they probably contain certain taxol or taxol analog.

Key concepts: Plant use of endophytic fungi in defense, Taxus, Bark (sound), High-performance liquid chromatography, Botany, Thin-layer chromatography, Fungus, Biology

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Separation and Identification of a New Taxus chinensis var. mairei Endophytic Fungus (Bionectria sp.) and the Activity of Its Metabolites — Research Paper | ScholarLens