Study of HPV infection in cervical adenocarcinoma and relation to expressions of Skp2 and p27~(kip1) protein

[Objective] To study the roles of S-phase kinase-associated protein2 (Skp2) and cell cycle regulator p27kip1 in occurrence and development of cervical adenocarcinoma, and to analyze the relationship between the infection of human papilloma virus (HPV) and the expressions of Skp2 and p27kip1 protein. [Methods] The expressions of HPV16/18DNA and Skp2, p27kip1 protein were determined by tissue microarray combined with in situ hybridization and immunohistochemistry in 86 cases of cervical adenocarcinoma and 24 cases of cervical chronic inflammation. [Results] The positive rates of HPV16/18DNA and Skp2 protein in cervical adenocarcinoma were 65.1% and 68.6% respectively, which significantly higher than that in cervical chronic inflammation(P 0.01). The positive rate of p27kip1 protein in cervical adenocarcinoma was 55.8%, which significantly lower than that in cervical chronic inflammation(P 0.05). There was no relationship between HPV16/18 infection and the pathological grades or histological subtypes, otherwise, there was positive correlation between HPV16/18 infection and Skp2 expression (P 0.05), there is negative correlation between HPV16/18 infection and p27kip1 expression. The expression of Skp2 was related to the pathological grades, the positive rate of Skp2 was significantly higher in G2 and G3 than in G1(P 0.05). The expressions of Skp2 and p27kip1 were related to the histological types. [Conclusion] Carcinogenesis of cervical adenocarcinoma may be related to the infection of HPV16/18 and the abnormal expressions of Skp2 and p27kip1. Their synergistic reaction may lead to the development of cervical adenocarcinoma.