Expression of Skp2 and p27(kip1) in cervical adenocarcinoma

Objective:To test the expressions of S phase kinase-associated protein 2 (Skp2) and cell cycle regulator p27 (kip1) in cervical adenocarcinoma. Methods:The expressions of Skp2 and p27 (kip1) were determined by tissue microarray combined with immunohistochemistry in 106 cases of cervical adenocarcinoma and 22 cases of cervical chronic inflammation. Results:The positive rate of Skp2 in cervical adenocarcinoma was 67.9%, which was significantly higher than that in cervical chronic inflammation(P0.01). The positive rate of p27(kip1) in cervical adenocarcinoma was 58.5%, which was markedly lower than that in cervical chronic inflammation(P0.05). The positive expression of Skp2 was significantly higher in G_2 and G_3 stage than in G_1 stage of cervical adenocarcinoma(P0.05). The expressions of Skp2 and p27(kip1) were related to the histologic classification. The level of Skp2 was significantly higher in endometrioid adenocarcinoma than that in other types(P0.05). The level of p27(kip1) was significantly higher in clear-cell adenocarcinoma than that in other types. There was no obviously negative correlation between the expression of Skp2 and p27 (kip1) in cervical adenocarcinoma. Conclusions:High expression of Skp2 and low expression of p27 (kip1) play an important role in the progression of cervical adenocarcinoma. Skp2 might be the one of biomolecular markers of malignancy for cervical adenocarcinoma. Tissue microarray is reliable and practical to detect the expressions of Skp2 and p27 (kip1).