Relationship between the Infection of HPV16/18 and Expression of Ki67 and p53 Protein in Cervical Adenocarcinoma

Objective To study the expression of Ki67 and p53 proteins in cervical adenocarcinoma,and to analyze the relationship between the infection of human papillomavirus 16,18(HPV16/18) and the expressions of ki67 and p53 proteins.Methods The expression of HPV16/18-E6DNA,Ki67 and p53 proteins were determined by tissue microarray combined with in situ hybridization and immunohistochemistry in 86 cases of cervical adenocarcinoma and 24 cases of cervical chronic inflammation.Results The positive rates of HPV16/18-E6DNA,Ki67 and p53 proteins in cervical adenocarcinoma were 65.1%,51.2% and 45.3% respectively,which were significantly higher than that in cervical chronic inflammation(8.3%,0 and 0,P0.01).HPV16/18 infection was positively correlated with Ki67 expression and negatively correlated with p53 expression.No correlation between the HPV16/18 infection and the pathological grades or histological subtypes existed in cervical adenocarcinoma.The expression of Ki67 and p53 proteins was correlated to the histological subtypes,with significantly higher expression rate in G2 and G3 tumors than that in G1 tumors.Conclusion HPV16/18 infection and abnormal expression of ki67,p53 proteins might be involved in the carcinogenesis and development of cervical adenocarcinoma.