Optimization of Proanthocyanidin Extraction from Grape Seed

The aim of this study was to optimize technical conditions for ultrasonic-assisted ethanol extraction,cellulase hydrolysis-based extraction and microwave-assisted ethanol extraction of proanthocyanidins from grape seed power using one-factor-at-a-time coupled with orthogonal array design method.Microwave-assisted extraction was found to be the best of the three methods,and the optimal process conditions were ethanol concentration of 80%,material-to-liquid ratio of 1:25(g/mL),microwave treatment time of 40 s and microwave power of 115 W.Under these conditions,the extraction rate of proanthocyanidins was 10.70%.