Chemical constituents from the seeds of Castanea mollissima Blume(III)

Objective To study the chemical constituents from the seeds of Castanea mollissima Blume and elucidate their structures.Methods The compounds were isolated by column chromatography on silica gel,Al2O3 columns,together with recrystallization methods.NMR,ESI-MS spectral analysis and TLC were employed for the structural identification.Results Six compounds were obtained and elucidated as:p-hydroxybenzoic acid(1),protocatechuic acid(2),gallic acid(3),daucosterol-6′-linoleate(4),ergost-6,22-3β-5α-8α-triol(5),3′,3″-dimethoxylarreatricin(6).Conclusions Among these compounds,compounds 46 were obtained from the Castanea genus for the first time.