Isolation and identification of chemical constituents from testa of Castanea mollissima Blume

Objective To study the chemical constituents from the testa of the Castanea mollissima Blume and elucidate their structures.Methods The compounds were isolated by column chromatography on silica gel,Al2O3 columns,together with recrystallization methods.NMR spectral analysis and physical-chemical properties were employed for the structural identification along with the data in the reference.Results Eight compounds were obtained and elucidated as hexadecanoic acid-2,3-dihydroxypropyl ester(1),scoparone(2),eupatilin(3),jaceosidin(4),5,7,4′-trihydroxy-6,3′,5′-trimethoxyflavone(5),clovane-2,9-diol(6),β-sitosterol(7),daucosterol(8),respectively.Conclusions Among them,compounds 36 are obtained from the Castanea genus for the first time.