Cloning and Sequence Analysis of Growth Hormone Release Hormone(GHRH) Mature Peptide from Guangxi Bama Minipig

Objective To clone and analysis the sequence of GHRH mature peptide gene of Guangxi Bama minipig.Methods The sequence of GHRH exon3 was amplified by a specific pair of primers using Guangxi Bama minipig genome as template.After sequencing the GHRH exon3,the GHRH mature peptide was cloned by over-bang extension PCR protocol and then ligated into sub-clone vector for sequencing and restriction analysis.Results It was showed that the cloned GHRH exon3 and mature peptide gene from Guangxi Bama minipig contained 132 and 105 bp,respectively.Conclusion Sequencing confirmed that the sequence of GHRH exon3 and mature peptide of Guangxi Bama minipig were successfully cloned in the study.