cDNA Cloning and Sequence Analysis of Myostatin Gene from Guangxi Bama Minipig

The total RNA was purified from Guangxi Bama minipig muscle tissue.The complementary DNA(cDNA) encoding Myostatin protein was amplified by the reverse transcription polymerase chain reaction(RT-PCR) method.The amplified cDNA fragment was ligated into pMD18-T vector after purification.The recombinant was verified by the method of PCR,restrictive cleavage and sequencing,respectively.The result showed that the Bama minipig Myostatin gene cDNA was successfully cloned in this study.The homologous analysis indicated that the homologous rate was 99.7% compared with the porcine Myostatin gene reported in GenBank and this gene has high conservativeness among mammalian animals(92.3%).Two synonymous mutations and one missense mutation were detected in Myostatin gene cDNA sequence of Bama minipig.