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Cloning and Sequence Analysis of Ghrelin cDNA from Guangxi Bama Minipig

Aide Wang

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Abstract

Objective To clone and analyze the sequence of Ghrelin cDNA of Guangxi Bama minipig.Methods The sequence of Ghrelin cDNA was amplified by a specific pair of primers using Guangxi Bama minipig belly total RNA as template.The Ghrelin cDNA was cloned by RT-PCR and then ligated into pMD18-T vector after purification.The recombined pMD18-T vector was transformed into the coliform DH5α for sequencing.Results The cloned Ghrelin cDNA gene from Guangxi Bama minipig contained 357 bp.The homology analysis showed that compared with the sequence of other pig breeds reported in GenBank,the homology rate of the Ghrelin cDNA sequence were 99.7%.Conclusion Sequencing confirmed that the sequence of Ghrelin cDNA of Guangxi Bama minipig was successfully cloned in the study.

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What this paper is about

Objective To clone and analyze the sequence of Ghrelin cDNA of Guangxi Bama minipig.Methods The sequence of Ghrelin cDNA was amplified by a specific pair of primers using Guangxi Bama minipig belly total RNA as template.The Ghrelin cDNA was cloned by RT-PCR and then ligated into pMD18-T vector after purification.The recombined pMD18-T vector was transformed into the coliform DH5α for sequencing.Results The cloned Ghrelin cDNA gene from Guangxi Bama minipig contained 357 bp.The homology analysis showed that compared with the sequence of other pig breeds reported in GenBank,the homology rate of the Ghrelin cDNA sequence were 99.7%.Conclusion Sequencing confirmed that the sequence of Ghrelin cDNA of Guangxi Bama minipig was successfully cloned in the study.

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Available abstract

Objective To clone and analyze the sequence of Ghrelin cDNA of Guangxi Bama minipig.Methods The sequence of Ghrelin cDNA was amplified by a specific pair of primers using Guangxi Bama minipig belly total RNA as template.The Ghrelin cDNA was cloned by RT-PCR and then ligated into pMD18-T vector after purification.The recombined pMD18-T vector was transformed into the coliform DH5α for sequencing.Results The cloned Ghrelin cDNA gene from Guangxi Bama minipig contained 357 bp.The homology analysis showed that compared with the sequence of other pig breeds reported in GenBank,the homology rate of the Ghrelin cDNA sequence were 99.7%.Conclusion Sequencing confirmed that the sequence of Ghrelin cDNA of Guangxi Bama minipig was successfully cloned in the study.

Key concepts: Complementary DNA, Bama, Ghrelin, Cloning (programming), Biology, GenBank, Sequence analysis, Homology (biology)

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Cloning and Sequence Analysis of Ghrelin cDNA from Guangxi Bama Minipig — Research Paper | ScholarLens