Expression and clinical significance of TGF-β1 in placenta and plasma of preeclampsia

Objective To investigate the expression of TGF-β1 in placenta and serum from normal pregnant women and preeclampsia, so as to probe the possible mechanism of preeclampsia. Methods Placentas and serum were collected from 40 preeclampsia women including 22 mild preeclampsia and 18 severe preeclampsia as study group and 30 normal pregnant women as control group. The levels of TGF-β1 in placenta were detected by Western blot. Cellular location was determined by immunohistochemistry. The levels of TGF-β1 in serum were detected by enzyme linked immunosorbent assay. Results(1)The expressions of TGF-β1 protein was located in the trophoblast cell and also can be found in exuviate membrane organization. in placental villous syncytiontrophoblast of preeclampsia group were significantly higher than that of control group.(2)The results of Western Blot showed the expressions of TGF-β1 in severe and mild preeclampsia group were both significantly higher than that of control group(P0.01), and was higher in severe group than that in mild one.(P0.05).(3) The level of TGF-β1 in serum of the the mild preeclampsia group and the sever group were significantly higher than that of control group(P0.01). and was higher in severe group than that in mild one.(P0.05).(4) There were positively correlation between the expressions of TGF-β1 in placental and the level of TGF-β1 in serum of preeclampsia group. Conclusion TGF-β1 not only participated in pathogenic process of preeclampsia but also were related to its degree.