Simultaneous Determination of Four Effective Components in Huaijiao Pills by RP-HPLC

OBJECTIVE To develop a RP-HPLC method for the determination of sophoricosid,baicalin,genistein and wogonin in Huaijiao pills.METHODS A Diamonsil C18 column(4.6 mm×200 mm,5μm) was used at 30 ℃.The mobile phase consisted of acetonitrile-0.05% phosphoric acid aqueous under gradient elution(0-10 min,25%-37% acetonitrile;10-25 min,37%-60% acetonitrile).The flow rate was set at 1.0 mL·min-1.The detection wavelength was 260 nm.RESULTS The linearities ranged in 11.6-116 μg·mL-1 for sophoricosid(r=0 999 6,n=6),33.5-335 μg·mL-1 for baicalin(r=0.999 8,n=6),0.46-4.63 μg·mL-1 for genistein(r=0.999 8,n=6),1.54-15.4 μg·mL-1 for wogonin(r=0.999 7,n=6) respectively.Recoveries were 99.3% with RSD 1.1% for sophoricosid,99.6% with RSD 1.1% for baicalin,99.6% with RSD 1.4% for genistein,99.0% with RSD 0.8% for wogonin.CONCLUSION The method is quick,simple and repeatable for simultaneous determination of sophoricosid,baicalin,genistein and wogonin in Huaijiao pills.