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2014•Journal of Shihezi UniversityRequires access

Study on the Separation and Purification of Elaeagnus Angustifolia Polysaccharide by Using the DM-18 Macroporous Resins

Yang Gyu Ku

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Abstract

To research and optimize the best conditions of the DM-18 macroporous resins,a dynamic absorption and desorption experiment was designed.The result showed that the best conditions as follows:2.0 mg /mL of concentration and 7.0 of pH of the solution of Elaeagnus angustifolia polysaccharide,the sample loading amount of 3.0 BV,1.5 BV/h of the rate,the eluent concentration of 35%,the total quantity of eluent of 4.0 BV and eluent rate of 1.0 BV/h.The rates of absorption and desorption could achieve 90.13% and 92.17% under the conditions above.It concludes that the DM-18 macroporous resins is a good material to separae and purificate the Elaeagnus angustifolia polysaccharide.

About this research paper

What this paper is about

To research and optimize the best conditions of the DM-18 macroporous resins,a dynamic absorption and desorption experiment was designed.The result showed that the best conditions as follows:2.0 mg /mL of concentration and 7.0 of pH of the solution of Elaeagnus angustifolia polysaccharide,the sample loading amount of 3.0 BV,1.5 BV/h of the rate,the eluent concentration of 35%,the total quantity of eluent of 4.0 BV and eluent rate of 1.0 BV/h.The rates of absorption and desorption could achieve 90.13% and 92.17% under the conditions above.It concludes that the DM-18 macroporous resins is a good material to separae and purificate the Elaeagnus angustifolia polysaccharide.

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Available abstract

To research and optimize the best conditions of the DM-18 macroporous resins,a dynamic absorption and desorption experiment was designed.The result showed that the best conditions as follows:2.0 mg /mL of concentration and 7.0 of pH of the solution of Elaeagnus angustifolia polysaccharide,the sample loading amount of 3.0 BV,1.5 BV/h of the rate,the eluent concentration of 35%,the total quantity of eluent of 4.0 BV and eluent rate of 1.0 BV/h.The rates of absorption and desorption could achieve 90.13% and 92.17% under the conditions above.It concludes that the DM-18 macroporous resins is a good material to separae and purificate the Elaeagnus angustifolia polysaccharide.

Key concepts: Desorption, Polysaccharide, Column chromatography, Chromatography, Chemistry, Absorption (acoustics), Adsorption, Absorption capacity

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Study on the Separation and Purification of Elaeagnus Angustifolia Polysaccharide by Using the DM-18 Macroporous Resins — Research Paper | ScholarLens