Different Concentrations of Leptin Acts on Osteoblast Proliferation and Expression of VEGF mRNA

Objective To observe the effect of leptin on osteoblasts proliferation and vascular endothelial growth factor(VEGF) expression.To provide a theoretical basis for the clinical treatment of bone repair and regeneration after injury.Methods Primary osteoblasts were cultured in vitro by enzymatic digestion.OD values in each group was measured by MTT method;the expression of VEGF mRNA was assessed by RT-PCR.Results The MTT showed that after 24h,OD values in all experimental groups rises with the increasing concentration of leptin;The RT-PCR showed that,VEGF mRNA in neonatal rat osteoblasts has a stable expression;Semi-quantitative analysis showed that with the rising of leptin concentrations,VEGF mRNA expression increased gradually.Conclusions Leptin can promote osteoblast proliferation;VEGF mRNA can express stability in osteoblasts;VEGF mRNA expression could be up-regulated by leptin in a dose-dependent manner.