Effect of leptin on VEGF expression in human umbilical vein endothelial cells

Objective:To investigate the effect of leptin on the expression of VEGF in cultured human umbilical vein endothelial cells (HUVECs). Method:The leptins in different concentrations were used to stimulate the primary cultured HUVECs and then detected the level that the HUVECs express the VEGF. Result:With the same time, the expression of VEGF protein and VEGF mRNA increased when the level of leptin increased; meanwhile, with the same concentration of leptin, the expression of VEGF protein and VEGF mRNA increased when the time extended. Both are significant when they are analyzed by the statistics. Conclusion: The expression of VEGF is concerned with the level of leptin and the time that the leptin acts on the HUVECs.