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2004•Chinese Journal of OsteoporosisRequires access

Hypoxia induces RT-PCR expression of osteoblast VEGF mRNA

Wang Zhiying

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Abstract

Objective To investigate the expression changes in Sprague-Dauley rat osteoblast VEGF mR-NA after hypoxia induction in vitro . Methods Osteoblasts were collected from the calvaria of fetal rat using type II collagenase, then exposed to hypoxia for different time. The levels of osteoblast VEGF mRNA were detected by RT-PCR.Results The expression of VEGF mRNA increased significantly at 6h after exposure to hypoxia, and reached peak at 12h, but decreased at 24 h. The level of VEGF mRNA was similar to that in the control group at 48h in hypoxia. Conclusion Hypoxia regulates the expression of osteobiast VEGF at transcription level.

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What this paper is about

Objective To investigate the expression changes in Sprague-Dauley rat osteoblast VEGF mR-NA after hypoxia induction in vitro . Methods Osteoblasts were collected from the calvaria of fetal rat using type II collagenase, then exposed to hypoxia for different time. The levels of osteoblast VEGF mRNA were detected by RT-PCR.Results The expression of VEGF mRNA increased significantly at 6h after exposure to hypoxia, and reached peak at 12h, but decreased at 24 h. The level of VEGF mRNA was similar to that in the control group at 48h in hypoxia. Conclusion Hypoxia regulates the expression of osteobiast VEGF at transcription level.

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Available abstract

Objective To investigate the expression changes in Sprague-Dauley rat osteoblast VEGF mR-NA after hypoxia induction in vitro . Methods Osteoblasts were collected from the calvaria of fetal rat using type II collagenase, then exposed to hypoxia for different time. The levels of osteoblast VEGF mRNA were detected by RT-PCR.Results The expression of VEGF mRNA increased significantly at 6h after exposure to hypoxia, and reached peak at 12h, but decreased at 24 h. The level of VEGF mRNA was similar to that in the control group at 48h in hypoxia. Conclusion Hypoxia regulates the expression of osteobiast VEGF at transcription level.

Key concepts: Calvaria, Hypoxia (environmental), Osteoblast, Messenger RNA, Collagenase, VEGF receptors, Chemistry, Angiogenesis

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