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2004•Journal of Sichuan UniversityRequires access

Identfication of Purity of the Hybrid Seed of Shuza No.9 (Brassica napus L.) with Random Amplified Polymorphic DNA Markers

Maolin Wang

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Abstract

Both maternal-specific and paternal-specific DNA bands were amplified with the DNA of parents of hybrid rapeseed Shuza No.9 by primer S18 and S31 which were selected from 10mer 50 RAPD primers analyzed in this study. Then the primers were used to amplify all the DNA of F_1 rapeseed and the results indicated that the specificity and stability of primer S18 and S31 was trusty.The hybrid seed purity of Shuza No.9 could be determined reliably with them.

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What this paper is about

Both maternal-specific and paternal-specific DNA bands were amplified with the DNA of parents of hybrid rapeseed Shuza No.9 by primer S18 and S31 which were selected from 10mer 50 RAPD primers analyzed in this study. Then the primers were used to amplify all the DNA of F_1 rapeseed and the results indicated that the specificity and stability of primer S18 and S31 was trusty.The hybrid seed purity of Shuza No.9 could be determined reliably with them.

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Available abstract

Both maternal-specific and paternal-specific DNA bands were amplified with the DNA of parents of hybrid rapeseed Shuza No.9 by primer S18 and S31 which were selected from 10mer 50 RAPD primers analyzed in this study. Then the primers were used to amplify all the DNA of F_1 rapeseed and the results indicated that the specificity and stability of primer S18 and S31 was trusty.The hybrid seed purity of Shuza No.9 could be determined reliably with them.

Key concepts: RAPD, Primer (cosmetics), Rapeseed, Brassica, DNA, Biology, Genetics, Molecular biology

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Identfication of Purity of the Hybrid Seed of Shuza No.9 (Brassica napus L.) with Random Amplified Polymorphic DNA Markers — Research Paper | ScholarLens