Fingerprints Construction of Hybrid Parents in Brassica Napus of the Series of Guiza and Its Utilization in Hybrid Purity Test

In the study,morphology method and the SSR marker method were used to analyze 43 brssica napus materials including Guiza series hybrid and their parents etc.Differences between the two methods were discussed.High multi-state property SSR primer were selected used in building DNA fingerprinting database of Guiza series hybrid and their parents.The main results were as follows.24 brassica napus inbred lines were analyzed by using SSR.Twenty-one pairs out of one hundred pairs of SSR primers were selected to give polymorphic,stable and repeated amplification profiles.Four primers including Na12C06,Na10B04b,0110A05,Na10D03 were selected to build DNA fingerprinting database of all 24 inbred lines.DNA fingerprint map of 19 brassica napus hybrids were built with 21 pairs SSR primes.These primers also gave polymorphic,stable and repeated amplification profiles.Four primers including Na12C06、Na10D03、Na10E02c、0110A05 were selected to build the DNA fingerprinting database of all 19 brassica napus hybrids.Using primer-in-group method can expand the core primers quickly to identify the purity and reality of brassica hybrids.