CE determination of chlorogenic acid,rutin and quercetin in Flos Lonicerae

Objective:To develop a CE method for determination of chlorogenic acid,rutin and quercetin in Flos Lonicerae.Methods:Chlorogenic acid,rutin and quercetin in Flos Lonicerae were extracted by microwave assisted technique,and then determined by capillary electrophoresis.A fused silica capillary column(64.5cm×50μm,an effective length of 56.0cm)was used.The running buffer was composed of 30 mmol·L~(-1)borax(pH=9.3).The applied voltage was 20 kV and the capillary temperature was 25℃.The detection wavelength was 340 nm for chlo- rogenic acid and 270 nm for rutin and quercetin.Results:The three components in Flos Lonicerae were separated successfully in 15 min.A good linearity between peak area and concentration was found in the range of 5-200 mg ·L~(-1)for the three components(r0.998).Their average recoveries were between 99.1%-102.7%,and the rel- ative standard deviation was less than 2.9%(n=5).Conclusion:The method is simple and rapid with satisfactory recoveries and good repeatability.It is likely to be a better way for quality control of Flos Lonicerae.