2009•Anhui nongye kexueRequires access
Simultaneous Determination of Chlorogenic Acid,Rutin and Luteolin in Flos Lonicerae by RP-HPLC
Hao Feng-xia
Open publisher page 0 citations
Abstract
[Objective] The aim of the study was to determine chlorogenic acid,rutin and luteolin contents in Flos Lonicerae simultaneously.[Method] With Zorbax SB-C18 column(250 mm×4.6 mm,5 μm),methanol-0.02% Phosphoric acid(50∶50)as mobile phase,flow velocity at 1.0 ml/min,detection wave length at 350 nm and column temperature at 30 ℃,the chlorogenic acid and the flavonoid compounds contents in Flos Lonicerae were determined by RP-HPLC.[Result] The chlorogenic acid,rutin and luteolin contents have obvious differences in different kinds of Flos Lonicerae.[Conclusion] The method is simple,feasible,and can be used to control the quality of Flos Lonicerae.