2002•Journal of Sun Yat-sen UniversityRequires access
Study on the Expression of bcl-2 Gene in PC12 Cells by Cloning and Transfection
Yongqing Wu, Liu Yan-wen, Pengxin Qiu, Jian Ge
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Abstract
Objective To investigate the expression of bcl-2 gene (bcl-2) in PC12 cell line. Methods The recombinant expression plasmid pcDNA3-bcl-2 was constructed from pSFFV-bcl-2, then it was introduced into PC12 cell line by liposome method. Western blotting and immunohistochemistry in situ were applied to exam the exogenous gene expression. Results The recombinant expression plasmid pcDNA3-bcl-2 was constructed successfully. In PC12 cell line transfected by the plasmid, 26 ku protein was expressed by Western-Blot test and immunoreactivity of cells were shown by immunohistochemical staining. Conclusion bcl-2 can be expressed effectively in PC12 cell lines transfected by recombinant plasmid pcDNA3-bcl-2. The success of this research provides us with the basis for further study on the bcl-2 function in PC12 cell line.