Study on the Transfection of the Apoptosis -Resistant Gene bcl-2 into Mammalian Cell

Objective: To construct the mammalian expression plasmid of bcl-2 gene, which is believed to have the effect of resisting the apoptosis, and transfect the mammalian cell with it. Methods: The full length cDNA of bcl-2 gene was put into expression plasmid pCMV-Tag 1 by gene recombination method. The 293T cell lines were transfected with the bcl-2 expression plasmid through the calcium phosphate coprecipitation technology. The expression of bcl-2 fusion protein was detected with the immunoprecipitation and Western blotting technology. Results: Full length cDNA of bcl-2 gene was constructed into the expression plasmid of pCMV-Tag 1 successfully. Bcl-2 fusion protein had been detected successfully in transfected 293T cell lines, and the size of the fusion protein was the same as expected. Conclusion: This study indicated that the expression plasmid can express in cultured mammalian cells and can be used to transfect the apoptosis-resistant gene bcl-2 into the islet cell. 口口