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2011•Journal of Tropical MedicineOpen access

Cloning of Bcl-xl gene and its transfection into SH-SY5Y cells

Qingyu Shen

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Abstract

Objective To study the expression of Bcl-xl gene in SH-SY5Y cells.Methods Plasmid pIRES2-EGFP/Bcl-xl was constructed and transfected into SH-SY5Y cells using Lipofectamine 2000.RT-PCR and Western-blot were employed to test expression of Bcl-xl.Results We successfully constructed pIRES2-EGFP/Bcl-xl.Transfection of the recombinant plasmid into SH-SY5Y cells resulted in a high level of expression of Bcl-xl RNA(by PCR) and the protein(by Western blot).Conclusion pIRES2-EGFP/Bcl-xl can be transfected and expressed in SH-SY5Y cells.The transfected cells can be used to study the effects of Bcl-xl on SH-SY5Y cells.

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Objective To study the expression of Bcl-xl gene in SH-SY5Y cells.Methods Plasmid pIRES2-EGFP/Bcl-xl was constructed and transfected into SH-SY5Y cells using Lipofectamine 2000.RT-PCR and Western-blot were employed to test expression of Bcl-xl.Results We successfully constructed pIRES2-EGFP/Bcl-xl.Transfection of the recombinant plasmid into SH-SY5Y cells resulted in a high level of expression of Bcl-xl RNA(by PCR) and the protein(by Western blot).Conclusion pIRES2-EGFP/Bcl-xl can be transfected and expressed in SH-SY5Y cells.The transfected cells can be used to study the effects of Bcl-xl on SH-SY5Y cells.

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Available abstract

Objective To study the expression of Bcl-xl gene in SH-SY5Y cells.Methods Plasmid pIRES2-EGFP/Bcl-xl was constructed and transfected into SH-SY5Y cells using Lipofectamine 2000.RT-PCR and Western-blot were employed to test expression of Bcl-xl.Results We successfully constructed pIRES2-EGFP/Bcl-xl.Transfection of the recombinant plasmid into SH-SY5Y cells resulted in a high level of expression of Bcl-xl RNA(by PCR) and the protein(by Western blot).Conclusion pIRES2-EGFP/Bcl-xl can be transfected and expressed in SH-SY5Y cells.The transfected cells can be used to study the effects of Bcl-xl on SH-SY5Y cells.

Key concepts: Transfection, Lipofectamine, Molecular biology, Western blot, SH-SY5Y, Bcl-xL, Plasmid, Biology

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