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2008•Journal of Jiangxi University of Traditional Chinese MedicineRequires access

Chromatographic Fingerprint of Luanpao Prescription by HPLC

BI Kai-shun

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Abstract

Objective: Chromatographic fingerprint analysis was established for the quality control of Luanpao Prescription(LPP).Methods: HPLC-UV fingerprint of LPP was developed with Zorbax SB C18 column and a simple linear gradient elution with the mobile phase consisted of acetonitrile-0.5% glacial acetic acid.The flow rate was set at 1.0 mL/min and the column temperature was kept at 30 ℃.The eluent was detected at 254 nm.The LC/MS method was applied for the identification of peaks in chromatographic fingerprint.Results: Fingerprint consisted of 15 common characteristic peaks in 10 different batches of normal samples.The similarity of CFP of 10 samples was over 0.938.Conclusion: The HPLC analysis of chromatography fingerprint could comprehensively control the quality of the formula.

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What this paper is about

Objective: Chromatographic fingerprint analysis was established for the quality control of Luanpao Prescription(LPP).Methods: HPLC-UV fingerprint of LPP was developed with Zorbax SB C18 column and a simple linear gradient elution with the mobile phase consisted of acetonitrile-0.5% glacial acetic acid.The flow rate was set at 1.0 mL/min and the column temperature was kept at 30 ℃.The eluent was detected at 254 nm.The LC/MS method was applied for the identification of peaks in chromatographic fingerprint.Results: Fingerprint consisted of 15 common characteristic peaks in 10 different batches of normal samples.The similarity of CFP of 10 samples was over 0.938.Conclusion: The HPLC analysis of chromatography fingerprint could comprehensively control the quality of the formula.

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Available abstract

Objective: Chromatographic fingerprint analysis was established for the quality control of Luanpao Prescription(LPP).Methods: HPLC-UV fingerprint of LPP was developed with Zorbax SB C18 column and a simple linear gradient elution with the mobile phase consisted of acetonitrile-0.5% glacial acetic acid.The flow rate was set at 1.0 mL/min and the column temperature was kept at 30 ℃.The eluent was detected at 254 nm.The LC/MS method was applied for the identification of peaks in chromatographic fingerprint.Results: Fingerprint consisted of 15 common characteristic peaks in 10 different batches of normal samples.The similarity of CFP of 10 samples was over 0.938.Conclusion: The HPLC analysis of chromatography fingerprint could comprehensively control the quality of the formula.

Key concepts: Chromatography, Fingerprint (computing), High-performance liquid chromatography, Gradient elution, Chemistry, Acetic acid, Chromatographic separation, Elution

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