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2015•Gansu Nongye Daxue xuebaoRequires access

Heterotic grouping of 96 inbred lines based on SSR markers in maize

Bai Yan

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Abstract

With the aim to solve the problem that the own-produced inbred lines in most breeding units commonly have low breeding efficiency for their confused genetic basis.In this study,70 SSR markers,which are uniformly existed in maize genome,were used to analyze the genetic diversity in a set of 96 maize inbred lines.The results showed that 70 primers produced 223 polymorphic amplified fragments,the average allele number per SSR locus was 3.61 with a range from 2to 5.The polymorphism information content(PIC)for the SSR loci varied from0.153 to 0.787 with an average of 0.60.Genetic similarities among the96 inbred lines ranged from 0.497 to 0.919 with an average of 0.640.The 96 maize inbred lines were divided into six heterotic groups,and could be simply merged into two groups(SS and Non-SS)by UPGMA.

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With the aim to solve the problem that the own-produced inbred lines in most breeding units commonly have low breeding efficiency for their confused genetic basis.In this study,70 SSR markers,which are uniformly existed in maize genome,were used to analyze the genetic diversity in a set of 96 maize inbred lines.The results showed that 70 primers produced 223 polymorphic amplified fragments,the average allele number per SSR locus was 3.61 with a range from 2to 5.The polymorphism information content(PIC)for the SSR loci varied from0.153 to 0.787 with an average of 0.60.Genetic similarities among the96 inbred lines ranged from 0.497 to 0.919 with an average of 0.640.The 96 maize inbred lines were divided into six heterotic groups,and could be simply merged into two groups(SS and Non-SS)by UPGMA.

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Available abstract

With the aim to solve the problem that the own-produced inbred lines in most breeding units commonly have low breeding efficiency for their confused genetic basis.In this study,70 SSR markers,which are uniformly existed in maize genome,were used to analyze the genetic diversity in a set of 96 maize inbred lines.The results showed that 70 primers produced 223 polymorphic amplified fragments,the average allele number per SSR locus was 3.61 with a range from 2to 5.The polymorphism information content(PIC)for the SSR loci varied from0.153 to 0.787 with an average of 0.60.Genetic similarities among the96 inbred lines ranged from 0.497 to 0.919 with an average of 0.640.The 96 maize inbred lines were divided into six heterotic groups,and could be simply merged into two groups(SS and Non-SS)by UPGMA.

Key concepts: Inbred strain, UPGMA, Biology, Locus (genetics), Genetic diversity, Genetics, Allele, Genetic variation

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