Isolation and Identification of Pseudorabies Virus HN Strain

One virus strain has been isolated from the samples of brain and visceral organs of a death piglet from a hogpen in Henan province.The virus was continually inoculated 5 generations onto PK15 cells and induced typical cytopathogenic effect(CPE).The typical pseudorabies virus particles could be found under electro-microscope.The isolate could be neutralized by the positive serum to pseudorabies virus.Rabbits and mice were injected with the isolate and the typical clinical syndrome could be observed.The sequence of 928 bases lying in gE gene could be amplified by the DNA extracted from the isolate.All of the evidences indicated that the isolated virus was pseudorabies virus and was named HN strain.