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Isolation and identification of pig pseudorabies virus strain GXA in Guangxi

Yi Xiong

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Abstract

One strain of virus was isolated from the neonatal pig of a farm in Guangxi. The virus was adapted to propagate in PK-15 cell and resulted in cytopathogenic effect (CPE). Pseudorabies viruses were observed under electronic microscope and the inoculated rabbits showed classical clinical signs when challenged with the culture. With the special primer, the gp50 about 217bp was obtained by PCR. According to the above results, this isolated virus is a pig pseudorabies virus.

About this research paper

What this paper is about

One strain of virus was isolated from the neonatal pig of a farm in Guangxi. The virus was adapted to propagate in PK-15 cell and resulted in cytopathogenic effect (CPE). Pseudorabies viruses were observed under electronic microscope and the inoculated rabbits showed classical clinical signs when challenged with the culture. With the special primer, the gp50 about 217bp was obtained by PCR. According to the above results, this isolated virus is a pig pseudorabies virus.

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Available abstract

One strain of virus was isolated from the neonatal pig of a farm in Guangxi. The virus was adapted to propagate in PK-15 cell and resulted in cytopathogenic effect (CPE). Pseudorabies viruses were observed under electronic microscope and the inoculated rabbits showed classical clinical signs when challenged with the culture. With the special primer, the gp50 about 217bp was obtained by PCR. According to the above results, this isolated virus is a pig pseudorabies virus.

Key concepts: Pseudorabies, Virology, Virus, Biology, Strain (injury), Primer (cosmetics), Inoculation, Guinea pig

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