Identification of a Swine Pseudorabies Virus Field Isolate

An isolate of pseudorabies virus(PRV) was obtained from the tissues of dead piglets from a farm with PRV-like disease in Henan province.This isolate of PRV could induce typical cytopathogenic effects after several passages on PK-15 cells and be neutralized by PRV positive serum in microneutralization test.This isolate was sensitive to chloroform and ether,and could be inactivated in 56 ℃ water bath after 30 min.After inoculation,both rabbits and mice showed typical clinical symptoms of pseudorabies.The cloning and sequencing of gp50 gene showed that the nucleotide homology of this isolate with other 5 PRV isolates previously published in GenBank was 99%,indicating that this isolate is PRV.