Determination of Icariin,Baohuoside I,Crocin-I and Crocin-II in Huichunjiu by HPLC

Objective: To develop an HPLC method for determination of the content of icariin, baohuoside I,crocin-Ⅰand crocin-Ⅱ in Huichunjiu(HCJ). Method: An Hypersil C 18 column was used as the chromatographic column,the flow rate was 0. 8 mL·min- 1. The mobile phase A consisted of acetonitrile,the mobile phase B consisted of 1% acetic acid solution,the UV detection wavelength was at 270 nm(for icariin and baohuoside I). The mobile phase A consisted of acetonitrile-methanol(2 ∶ 3),the mobile phase B consisted of 0. 5% phosphoric acid solution,the UV detection wavelength was at 440 nm(for crocin-Ⅰ and crocin-Ⅱ). Result: There were a good linear relationship between the concentration of icariin,baohuoside I and peak area value when the concentrations of icariin and baohuoside I were within the range of 0. 054 6-1. 092 μg(r = 0. 999 7), 0. 076 2-1. 524 μg(r = 0. 999 1). The average recovery were 97. 56%(RSD 0. 94%) and 99. 17%(RSD 0. 77%). There were a good linear relationship between the concentration of crocin-Ⅰ,crocin-Ⅱ and peak area value when the concentrations of crocin-Ⅰand crocin-Ⅱ were within the range of 0. 102 8-2. 056 μg(r = 0. 999 4), 0. 060 4-1. 208 μg(r = 0. 999 8). The average recovery were 97. 54%(RSD 0. 90%) and 96. 91%(RSD 1. 27%). Conclusion: The method is accurate,sensitive and reproducible,it may be used in the determination of icariin,baohuoside I,crocin-Ⅰ and crocin-Ⅱ in HCJ.