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2010•Journal of Nuclear Agricultural SciencesRequires access

ISOLATION OF mtDNA FRAGMENTS ASSOCIATED TO CMS IN HOT PEPPER BY AFLP TECHNIQUE

WU Zhi-ming, Kailin Hu

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Abstract

Mitochondrial DNA(mtDNA) from pepper CMS line 'North A',maintainer line 'North B' and their hybrid F_1 were analyzed and compared by AFLP technique.9 specific fragments were obtained,and were cloned and sequenced.BLAST analysis in GenBank revealed that partial sequences shared above 90% identity with NADH dehydrogenase subunit 1,ribosomal protein L2 of Nicotiana tabacum,nad4L-orf25 intergenic sequence of Petunia×hybrida mitochondrial DNA at nucleotide level.It was speculated that the specific fragments might be related to CMS in pepper.

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What this paper is about

Mitochondrial DNA(mtDNA) from pepper CMS line 'North A',maintainer line 'North B' and their hybrid F_1 were analyzed and compared by AFLP technique.9 specific fragments were obtained,and were cloned and sequenced.BLAST analysis in GenBank revealed that partial sequences shared above 90% identity with NADH dehydrogenase subunit 1,ribosomal protein L2 of Nicotiana tabacum,nad4L-orf25 intergenic sequence of Petunia×hybrida mitochondrial DNA at nucleotide level.It was speculated that the specific fragments might be related to CMS in pepper.

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Available abstract

Mitochondrial DNA(mtDNA) from pepper CMS line 'North A',maintainer line 'North B' and their hybrid F_1 were analyzed and compared by AFLP technique.9 specific fragments were obtained,and were cloned and sequenced.BLAST analysis in GenBank revealed that partial sequences shared above 90% identity with NADH dehydrogenase subunit 1,ribosomal protein L2 of Nicotiana tabacum,nad4L-orf25 intergenic sequence of Petunia×hybrida mitochondrial DNA at nucleotide level.It was speculated that the specific fragments might be related to CMS in pepper.

Key concepts: Mitochondrial DNA, GenBank, Biology, Pepper, Intergenic region, Genetics, NADH dehydrogenase, Nicotiana tabacum

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