Effect of lead acetate on growth of murine mesenchymal stem cells

Objective To explore the effect of lead acetate on the growth of murine mesenchymal stem cells in vitro.Methods 40.00,60.00 and 100.00 μmol·L-1 of lead acetate were used in the culture of colony-forming unit-fibroblast(CFU-F),the effect on the rate of colony-forming and the rule of variation were observed.Results The rates of colony-forming were(3.30±0.20),(2.40±0.10) and(1.57±0.21)/105,when the doses of lead acetate were 40.00,60.00 and 100.00 μmol·L-1 and there were significant differences compared with control group(4.20±0.20)/105,P0.05).The rates of colony-forming had significant differences between lead acetate with different doses groups(P0.05).Dose-effect relationship was analyzed with linear correlation and correlation coefficient(r=-0.960,P0.01).Colony cells of control group presented fusiform shape while cells of 40.0 μmol·L-1 of lead acetate group presented irregularity or round shape cultivated in vitro after 6 d.Conclusion The lead acetate suppresses the rate of colony-forming of CFUF significantly in vitro and the inhibitory effect increases with the increasing of doses of lead acetate.