Effect of lead acetate on the growth of bone marrow mesenchymal stem cells in immature male rats

Objective To explore the effect of lead acetate on the growth of immature male rats bone marrow mesenchymal stem cells and Clarify the toxicity mechanism of lead acting on bone marrow in physiology circumstance.Method Different doses lead acetate(15、30、60 mg/kg) were fed to immature male rats exposed orally.8 weeks later,The lead levels of femoral artery blood were measured.Rats were killed and bone marrow of the bilateral femur was cultured by density gradient centrifugation in vitro.The effects of lead acetate on the rate of colony formation(CFU-F)、cell morphology and cell growth of bone marrow mesenchymal stem cells and the rule of variation were observed.Result Comparing diferent doses groups with the control group,there were significant differences of blood lead(P 0.05) and dose-effect relationship demonstrates negative correlation(r = 0.983,P 0.01).With the increase of lead concentration the rate of colony formation(CFU-F) decreased in a dose-dependent relationship(r =-0.967,P = 0.000).Meanwhile,the differences of between diferent dosesgroups with the control group were remarkable(P 0.05).The IC50 of CFU-F colony was 60.00 μmol/L.The results of MTT revealed that the proliferation velocity of high-dose lead group was slower than that in the control group(P 0.05).Conclusion The data suggests the growth and morphology of BMSC were significantly inhibited by lead acetate,further,the inhibitory effect enhances with the increasing of lead acetate doses.