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2004•Research and Practice on Chinese MedicinesRequires access

Determination of Puerarin in Xinxuesunning Granules by HPLC

Xing Hai-yan

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Abstract

Objective To establish a HPLC method for determination of puerarin in Xinxuesunning Granules.Method Sim-pack CLC C_(18)column (150 mm×4.6 mm,5 μm)was used with methanol-0.1%phos horic acid(22:78)as a mobile phase.The detection wavelength was set at 250 nm.Result The linear range of puerarin was in the range of 10.728~53.64μg·mL~(-1)(r=0.999 5).,The average recovery is (99.97)%,RSD=1.55%.Conclusion This mothod is simple,accurate and reproducible.It can be used for the quality control of Xinxuesunning Granules.

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What this paper is about

Objective To establish a HPLC method for determination of puerarin in Xinxuesunning Granules.Method Sim-pack CLC C_(18)column (150 mm×4.6 mm,5 μm)was used with methanol-0.1%phos horic acid(22:78)as a mobile phase.The detection wavelength was set at 250 nm.Result The linear range of puerarin was in the range of 10.728~53.64μg·mL~(-1)(r=0.999 5).,The average recovery is (99.97)%,RSD=1.55%.Conclusion This mothod is simple,accurate and reproducible.It can be used for the quality control of Xinxuesunning Granules.

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Available abstract

Objective To establish a HPLC method for determination of puerarin in Xinxuesunning Granules.Method Sim-pack CLC C_(18)column (150 mm×4.6 mm,5 μm)was used with methanol-0.1%phos horic acid(22:78)as a mobile phase.The detection wavelength was set at 250 nm.Result The linear range of puerarin was in the range of 10.728~53.64μg·mL~(-1)(r=0.999 5).,The average recovery is (99.97)%,RSD=1.55%.Conclusion This mothod is simple,accurate and reproducible.It can be used for the quality control of Xinxuesunning Granules.

Key concepts: Puerarin, Chromatography, High-performance liquid chromatography, Chemistry, Linear range, Detection limit, Medicine, Pathology

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