2009•Anhui nongye kexueRequires access
Construction and Identification on Bait Vector of Prion(prp105-125 flaw) in Two-hybrid System of Yeast
Hao Zhuo
Open publisher page 0 citations
Abstract
The gene segment of prion(prp105-125 flaw) of mice was amplified,and it was transferred into bait vector pSos to constructed bait vector pSos-prp with prion(prp105-125 flaw)of two-hybrid system of yeast.The toxicity and self-activation ability of the expression product of recombinant plasmid in yeast cell were detected when it was transferred into yeast cdc25H.The sequence analysis results showed that the bait vector pSos-prp with prion(prp105-125 flaw)of twohybrid system of yeast was successfully established and its expression product had no toxicity and self-activation ability on yeast cdc25H.