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Construction of Ricin-B(RT-B) Gene Bait Vector and Verification of Self-Activation in Yeast Double-Hybrid System

Gao Hong-wei

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Abstract

The ricin-B gene was cloned into the pSos vector.After being verified by restriction enzyme digestion analysis,the recombinant bait vector pSos-RTB of yeast double-hybrid system was obtained.Bait and target plasmids were cotransformed into the cdc25H yeast strain.This result means it was not toxic to cdc25H and had no self-activation.

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What this paper is about

The ricin-B gene was cloned into the pSos vector.After being verified by restriction enzyme digestion analysis,the recombinant bait vector pSos-RTB of yeast double-hybrid system was obtained.Bait and target plasmids were cotransformed into the cdc25H yeast strain.This result means it was not toxic to cdc25H and had no self-activation.

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Available abstract

The ricin-B gene was cloned into the pSos vector.After being verified by restriction enzyme digestion analysis,the recombinant bait vector pSos-RTB of yeast double-hybrid system was obtained.Bait and target plasmids were cotransformed into the cdc25H yeast strain.This result means it was not toxic to cdc25H and had no self-activation.

Key concepts: Yeast, Ricin, Plasmid, Recombinant DNA, Vector (molecular biology), Gene, Biology, Restriction enzyme

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Construction of Ricin-B(RT-B) Gene Bait Vector and Verification of Self-Activation in Yeast Double-Hybrid System — Research Paper | ScholarLens