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Cloning of Prion(prp23-231) Gene of Mouse and Construction of Bait Vector in Yeast Double-hybrid System

Gao Hong-wei

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Abstract

The prion gene was amplified and cloned into the pSos plasmid.After being verified by endonucleases digestion analysis,the recombinant bait vector pSos-prp23-231 of yeast double-hybrid system was obtained.The prp23-231 domain of mouse was not toxic to cdc25H and had no self-activation.

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What this paper is about

The prion gene was amplified and cloned into the pSos plasmid.After being verified by endonucleases digestion analysis,the recombinant bait vector pSos-prp23-231 of yeast double-hybrid system was obtained.The prp23-231 domain of mouse was not toxic to cdc25H and had no self-activation.

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Available abstract

The prion gene was amplified and cloned into the pSos plasmid.After being verified by endonucleases digestion analysis,the recombinant bait vector pSos-prp23-231 of yeast double-hybrid system was obtained.The prp23-231 domain of mouse was not toxic to cdc25H and had no self-activation.

Key concepts: Cloning (programming), Plasmid, Gene, Yeast, Recombinant DNA, Biology, Vector (molecular biology), Restriction enzyme

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