Optimization of Ultrasonic-assisted Extraction of Polysaccharide from Garlic by Orthogonal Array Design

With the extraction yield of polysaccharide as index,the process conditions for extracting polysaccharides from garlic with ultrasonic-assisted method are optimized by orthogonal array design on the basic of single-factor experiment.The results show that the extraction temperature and ratio of material and liquid are the key factors that affect the extraction yield of polysaccharide.The optimum conditions for the extraction of polysaccharide are as follows:extraction temperature of 50℃,extraction duration of 40min,ratio of material and liquid 0f1∶40(g/mL)and ultrasound power of 350W.Under such conditions,the extraction yield of polysaccharide from garlic is 25.12%.The optimized extraction process is stable and reasonable and it could be an available assay for polysaccharide extraction from garlic.