2011•Lishizhen Medicine and Materia Medica ResearchRequires access

Extraction Process of Water-soluble Polysaccharide in Huangmo by the Method Orthogonal Design Optimization

Wang Shao-yan

Abstract

Objective To study the extraction process of the water-soluble polysaccharide in Huangmo.Methods The orthogonal design was adopted to research the effect of water volume,extraction time,extraction temperature,extraction times and their interactions.The standard curve was used to check the content of polysaccharide with galactose as standard sample,and the detection wavelength was 490nm.Results The optimum extraction conditions were as follows: extraction 3 times with water 150ml per 10g Huangmo powder,extraction time 2h,extraction temperature 95℃.The yield rate and content of polysaccharide were 11.6% and 8.11%,respectively.Conclusion The process is suitable for extraction of water-soluble polysaccharide in Huangmo.

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Key concepts: Extraction (chemistry), Polysaccharide, Chromatography, Yield (engineering), Water extraction, Process (computing), Hot water extraction, Volume (thermodynamics)