Optimization of processing condition on peptide of rapeseed meal protein

In this paper,the technology of enzymolysis rapeseed meal protein was improved and enzymolysis conditions were optimized.Rapeseed meal protein was hydrolysised by 2709 alkaline protease.The relationship between protein recovery rate and enzymatic hydrolysis condition,such as pH,enzyme concentration,hydrolysis time,enzymolysis temperature,substrate concentration,was determin-ed.Based on the above-mentioned measures,the condition of 2709 alkaline protease hydrolysis rapeseed meal protein was optimized by the response surface methodology.The results showed that the optimition hydrolysis condition was pH 11.5,hydrolysis temperature 50 ℃,substrate concertation 4%,hydrolysis time 173 min,enzyme concentration 5 778 U·g-1;in this condition,the protein recovery rate was about 87.18%.