Optimization of alkaline protease extraction technology of protein from rapeseed meal by response surface methodology

Rapeseed meal was by-products of pressing rapeseed oil and a good resource of protein extraction.Using rapeseed meal as material,the technology for extracting rapeseed meal protein with alkaline protease was discussed.Based on systematic study of effect of liquid to solid ratio,enzyme dosage,value of initial pH,reaction temperature and reaction time on extraction rate of protein,main effect factors were optimized and optimum technology was received.When reaction time was constantly 40min,the result demonstrated extraction rate of protein was 55.38% under optimum conditions namely that enzyme dosage 6500U/g,reaction temperature 45℃,value of initial pH11,ratio of liquid to solid 17∶1.