Study on expression of Decorin mRNA and p57~(KIP2) mRNA and their clinical significance in non-small cell lung cancer

Objective:To investigate the expression of Decorin mRNA and p57 KIP2 mRNA and analyze their relationship with pathogenesis and clinical characteristics in non-small cell lung cancer.Methods:Paraffin-embedded tissues from 59 cases of non-small cell lung cancer and 12 cases of normal lung tissues adjacent to tumor tissues were selected to make tissue chip, and expression of Decorin mRNA and p57 KIP2 mRNA were examined by in situ hybridization using labelled digoxigenin probes.Results:The expression rates of Decorin mRNA were 20.3%(12/59) in tumor tissues and 66.7%(8/12) in normal tissues, The normal tissues showed a higher expression of Decorin mRNA than that of the tumor tissues(P0.05). The expression rate of Decorin mRNA was higher in large cell lung adenocarcinoma(LCLC) than that of squamous cell carcinoma of the lung(LSCC), The positive expression rates were 5.3%(1/19), 50%(4/4) respectively(P0.05). The expression rate of p57~(KIP2) mRNA in LSCC was 63.2%(12/19), being higher than adenocarcinoma of the lung(LAC), LCLC and alveolar carcinoma (P0.05). In non-small cell lung cancer without lymph node metastasis, The expression of Decorin mRNA and p57~(KIP2) mRNA was increased significantly(P0.05).However there was no correlation between Decorin mRNA and p57~(KIP2) mRNA.Conclusion:These results suggest that Decorin gene and p57 KIP2 gene closely relate to the non-small cell lung cancer metastasis and histological classification. The two genes may be an index in monitoring the status and evaluating the prognosis of the non-small cell lung cancer.