Rapid determination of 5 kinds of organic acid in Flos Lonicerae extracts by UV spectroscopy

Objective: A UV method was established for the rapid determination of 5 kinds of organic acids in Flos Lonicerae extracts.Methods:The HPLC method was used as the reference method to determine the contents of chlorogenic acid,caffeic acid,3,4-dicaffeoylquinic acid,3,5-dicaffeoylquinic acid and 4,5-dicaffeoylquinic acid in the Flos Lonicerae extracts.UV absorption spectrum was scanned at the 220-400 nm wavelength range.The PLS models was developed to correlate the UV spectra with the corresponding values determined by the reference method.Then the performance of calibration models was validated.Result:The correlation coefficients of the validation set were all above 0.90,and the relative predicted errors for the 5 kinds of organic acid were 1.46%,-2.13%,0.42%,6.40% and 3.83%,respectively.Conclusion: Owing to its simple,fast and accurate properties,the presented method could be a method for the rapid determination of organic acids in Flos Lonicerae extracts.