2001•Journal of Practical Aesthetic and Plastic SurgeryRequires access

Effects of PGE_2 on type I and III procollagen mRNA expression in scar-derived fibroblasts

Lin Zi-ha

Abstract

Objective The mechanisms of inhibitory effects of PGE 2 on collagen synthesis in scar derived fibroblasts was investigated.Methods Fibroblasts was isolated from fresh hypertrophic tissue and cultured, exposed to PGE 2. The concentration of type Ⅰ and Ⅲ procollagen mRNA expression in scar-fibroblasts were assayed by Dot blot hybridization. Results PGE 2 significantly reduced type Ⅰ and Ⅲ procollagen mRNA expression in scar-fibroblasts. Conclusion PGE 2 inhibits collagen synthesis of scar-fibroblasts at transcriptional level.

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Key concepts: Procollagen peptidase, Hypertrophic scar, Dot blot, Messenger RNA, Molecular biology, Chemistry, Western blot, Fibroblast