Effect of Resveratraol on Collagen Synthesis and Gene Expression

Objective To study the effects of Resveratraol on viability of fibroblasts and type Ⅰand Ⅲ collagen syntheses and degradation in hypertrophic scars.Methods (1)Various concentrations of Resveratroal were added into the medium and co-incubated with FB for different time period.MTT assay was employed to detect proliferation of fibroblast;(2)With Resveratraol was performed on hypertrophic scars.On the 3~(rd) day and 7~(th) day,type Ⅰ and Ⅲ procollagen mRNA expression were studied by means of immunohistochemistry and molecular biology.Results(1)The resveratraol in various concentrations inhibited proliferation of fibroblast;(2)On the 3~(rd) day after with Resveratraol,both type Ⅰ and Ⅲ procollagen mRNA expressions were significantly inhibited and on the 7~(th) day both procollagen mRNA expressions decreased further.With Resveratraol may inhibit type Ⅰ procollagen mRNA expression more than type Ⅲ.Conclusions Resveratraol may inhibit viability of fibroblasts and type Ⅰ and Ⅲ procollagen mRNA expression.