Cells Culture in Vitro of Adult Degenerated Nucleus Pulposus and Its Morphological Observation

Objective This study is to explore the disc degeneration influenced by the cell effects by cells culture of adult degenerative lumbar nucleus pulposus disc.Methods Five discs were obtained from five adults with disc herniation disease by perations.They were dissected and were digested with trypsin and collagenase.The isolated cells were cultured in DMEM/12.The cultured cells were identified with morphological observation and semiquantitative RT-PCR etc.Results The NPCs can be cultured in vitro,After 30 days it just can be subcultured.The proper culture conditions include bulk percentum of calf cerum and PH value of DMEM.The cultured NPCs show high expressions of collagen Ⅰand low expression of collagen Ⅱ.Conclusion NPCs culture in vivo the culture need longer time,also the proliferous ability is lower.where the shoe pinches is to explore the cell culture conditions.