Cells Culture of Rabbit Lumbar Nucleus Pulposus and Its Morphological Observation

Objective:To observe the continuous changes and explore the biological action and affecting factors of nucleus pulposus cells. Methods:Lumbar nucleus pulposus cells were cultured in F12 DMEM medium and 10% fetal bovine serum. We want to set up a cells culturing model,observe them through light microscope and electron microscope and assess the cells viability. Results: The biological characteristics of the primary generation of the nucleus pulposus cells were similar to those of the cells in vivo. After subculture, the cells are becoming senility. The assessment of the cell viability shows that the assessment are declined as the time of the culture passed. The structures of the organellae in nucleus pulposus cells varied with the changes of cell biological characteristics. Conclusion:This study is a basic work for culturing human degenerative nucleus pulposus cells.