Preparation of bovine and caprine placental peptide and studies on its biological activity

Purpose To search for a new immunoregulator and to utilize animal placenta adequately.MethodsThe distilled bovine and caprine placental peptides were prepared by ultrafiltering and their relative molecular weights were mensurated by improved Tricine-SDS-PAGE eletrophoresis with urea.Their absorbance peaks were got by ultraviolet scanning.Their immnoregulatory effects were mensurated by MTT colorimetric assay after establishing a model of in vitro immunosuppressive lymphocytes of rabbit.ResultsThe distilled bovine and caprine placental peptides were prepared successfully.Their relative molecular weights were 4 785 and 4 386 respectively.And their absorbance peaks were 210.5 nm and 225.4 nm respectively.The bovine and caprine placental peptides could improve immunosuppressive T lymphocyte transformation rate.The immunosuppressive lymphocyte transformation rates in groups with the bovine placental peptide of 1∶1×10~3 and 1∶1×10~4(P(0.05))and with the caprine placental peptides of 1∶1×10~2,1∶1×10~3and 1∶1×10~4(P0.01)increased more remarkably than that in immunosuppression group.ConclusionThere were placental peptides in bovine and caprine placenta which could be distilled successfully.And they could improve immunosuppressive T lymphocyte transformation rate remarkably,and may be a reasonable immunoregulator.